> For the complete documentation index, see [llms.txt](https://docs.hits.ai/hyperlab-use-case/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://docs.hits.ai/hyperlab-use-case/use-case/tp53-knockout-protocol-design-in-hek293t.md).

# 004. TP53 knockout protocol design in HEK293T

An end-to-end TP53 knockout protocol for HEK293T cells, built through an iterative, decision-driven workflow.

## Use Case in HyperLab

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<div align="center"><figure><img src="https://4290995292-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2Fc8jFSx45v33BUSaQuAOW%2Fuploads%2FvN9IRGfvcJMmXdx5xL3b%2Fuc-53cc013f3694f3f09265668029a3.png?alt=media" alt="27.png"><figcaption></figcaption></figure></div>

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<div align="center"><figure><img src="https://4290995292-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2Fc8jFSx45v33BUSaQuAOW%2Fuploads%2F15nuGC6cf8bYIJA7n1df%2Fuc-77125e2b75a4f95cd20fce7e0159.png?alt=media" alt="30.png"><figcaption></figcaption></figure></div>

<div align="center"><figure><img src="https://4290995292-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2Fc8jFSx45v33BUSaQuAOW%2Fuploads%2FMx4MKLFA4Coz1ru8caNX%2Fuc-392fe3d44ea7cd2f978bd53d6d41.png?alt=media" alt="32.png"><figcaption></figcaption></figure></div>

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<div align="center"><figure><img src="https://4290995292-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2Fc8jFSx45v33BUSaQuAOW%2Fuploads%2F4I1pUhxLrEYmhiYcQI8p%2Fuc-8e81b9106e9133ab7b440c044dbb.png?alt=media" alt="34.png"><figcaption></figcaption></figure></div>

## Artifacts

<div align="center"><figure><img src="https://4290995292-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2Fc8jFSx45v33BUSaQuAOW%2Fuploads%2FPYF6lDku9xLbGW0yHaBE%2Fuc-25c250c25339a5c222e04f7b5a33.png?alt=media" alt="tp53_knockout_workflow_illustrated_preview.png"><figcaption></figcaption></figure></div>